methanol activated immuno blot pvdf membrane Search Results


polyvinylidene fluoride membrane  (Bio-Rad)
96
Bio-Rad polyvinylidene fluoride membrane
Polyvinylidene Fluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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immuno blot pvdf membranes  (Bio-Rad)
96
Bio-Rad immuno blot pvdf membranes
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Immuno Blot Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
immuno blot pvdf membranes - by Bioz Stars, 2026-07
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immun blot pvdf bio rad  (Bio-Rad)
96
Bio-Rad immun blot pvdf bio rad
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Immun Blot Pvdf Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
immun blot pvdf bio rad - by Bioz Stars, 2026-07
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polyvinylidene difluoride membrane pvdf  (Bio-Rad)
94
Bio-Rad polyvinylidene difluoride membrane pvdf
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Polyvinylidene Difluoride Membrane Pvdf, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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polyvinylidene fluoride pvdf membrane  (Bio-Rad)
99
Bio-Rad polyvinylidene fluoride pvdf membrane
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Polyvinylidene Fluoride Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
polyvinylidene fluoride pvdf membrane - by Bioz Stars, 2026-07
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im muno bcip nbt liquid substrate plus  (Valiant Co Ltd)
95
Valiant Co Ltd im muno bcip nbt liquid substrate plus
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Im Muno Bcip Nbt Liquid Substrate Plus, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa abts murine kit  (PeproTech)
90
PeproTech elisa abts murine kit
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Elisa Abts Murine Kit, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa abts murine kit - by Bioz Stars, 2026-07
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immun-diagnostik enzyme-immuno-assay (eia)  (Immundiagnostik AG)
90
Immundiagnostik AG immun-diagnostik enzyme-immuno-assay (eia)
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Immun Diagnostik Enzyme Immuno Assay (Eia), supplied by Immundiagnostik AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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iris immune  (Immunome Inc)
86
Immunome Inc iris immune
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Iris Immune, supplied by Immunome Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clinically used concentrates albuminr  (Pharmacia Upjohn LLC)
90
Pharmacia Upjohn LLC clinically used concentrates albuminr
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Clinically Used Concentrates Albuminr, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human immune response 25 copyright 2023 immunome  (Immunome Inc)
86
Immunome Inc human immune response 25 copyright 2023 immunome
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Human Immune Response 25 Copyright 2023 Immunome, supplied by Immunome Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immuno-oncology biomarker working  (International Immuno Diagnostics Inc)
90
International Immuno Diagnostics Inc immuno-oncology biomarker working
Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to <t>PVDF</t> membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).
Immuno Oncology Biomarker Working, supplied by International Immuno Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to PVDF membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).

Journal: Aging (Albany NY)

Article Title: Age-associated alterations in inducible gene transcription in human CD4 + T lymphocytes

doi:

Figure Lengend Snippet: Purified CD4 + T cells were incubated at 37°C with plate-bound anti-CD3 for 2h or 4h; control cells were incubated for 4h without additional treatment. ( A ) Nuclear extracts (NE) were fractionated by SDS-PAGE, transferred to PVDF membranes and probed with antibodies directed against p65/RelA, transcription factors SP1 and TATA binding protein (TBP), or β actin. p65/RelA levels from 3 donors were compared to varying concentrations of a control cytoplasmic extract (CE). Induced p65/RelA levels in all 29 donors are shown in . ( B ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 29 donors after 2h or 4h activation separated by subjects less than 65 years (Y) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in Y and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in young 0.41; 0.52) (in old 0.38; 0.39). ( C ) Fold-increase of nuclear p65/RelA treated cells was calculated relative to the levels in untreated cells . The average fold increase of nuclear p65/RelA for 21 donors after 2h or 4h activation separated by subjects 50-64 years (M) and 65 and over (O) is shown in the graph. p -values shown above bar graphs compare the fold induction between 2h and 4h of activation in M and O groups, and were determined by Student's test ( p <0.05 was considered as significant). Error bars reflect the standard error of the mean (±SEM) (in middle 0.66; 0.83) (in old 0.38; 0.39).

Article Snippet: Extracts were fractionated by electrophoresis through 10% SDS-poly-acrylamide gels, transferred to Immuno-Blot PVDF membranes (Bio-Rad) and probed with primary antibody [NF-κB p65 (C-20) rAB at 1:1000 (Santa Cruz), Sp1 (H-225) rAB at 1:1000 (Santa Cruz), TBP mAB at 1:500 (Abcam), beta actin mAb at 1:1000 (Abcam)].

Techniques: Purification, Incubation, Control, SDS Page, Binding Assay, Activation Assay